Electron microscopy of rabbit FC crystals
| العنوان: | Electron microscopy of rabbit FC crystals |
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| المؤلفون: | Wah Chiu, Laura L. Degn, Robert A. Grant, John Robinson |
| المصدر: | Proceedings, annual meeting, Electron Microscopy Society of America. 41:730-731 |
| بيانات النشر: | Cambridge University Press (CUP), 1983. |
| سنة النشر: | 1983 |
| مصطلحات موضوعية: | Crystallography, Materials science, law, Rabbit (nuclear engineering), General Medicine, Electron microscope, law.invention |
| الوصف: | Proteolytic digestion of the immunoglobulin IgG with papain cleaves the molecule into an antigen binding fragment, Fab, and a compliment binding fragment, Fc. Structures of intact immunoglobulin, Fab and Fc from various sources have been solved by X-ray crystallography. Rabbit Fc can be crystallized as thin platelets suitable for high resolution electron microscopy. The structure of rabbit Fc can be expected to be similar to the known structure of human Fc, making it an ideal specimen for comparing the X-ray and electron crystallographic techniques and for the application of the molecular replacement technique to electron crystallography. Thin protein crystals embedded in ice diffract to high resolution. A low resolution image of a frozen, hydrated crystal can be expected to have a better contrast than a glucose embedded crystal due to the larger density difference between protein and ice compared to protein and glucose. For these reasons we are using an ice embedding technique to prepare the rabbit Fc crystals for molecular structure analysis by electron microscopy. |
| تدمد: | 2690-1315 0424-8201 |
| DOI: | 10.1017/s0424820100077220 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::63b263299afdd4fe0fd53471eae9b7e3 https://doi.org/10.1017/s0424820100077220 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........63b263299afdd4fe0fd53471eae9b7e3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 26901315 04248201 |
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| DOI: | 10.1017/s0424820100077220 |