Isolation and characterization of thermostable phycocyanin from Galdieria sulphuraria
| العنوان: | Isolation and characterization of thermostable phycocyanin from Galdieria sulphuraria |
|---|---|
| المؤلفون: | William I. Suh, Myounghoon Moon, Chul Woong Kim, Sanjiv K. Mishra, Ji-Won Yang, Min S. Park |
| المصدر: | Korean Journal of Chemical Engineering. 31:490-495 |
| بيانات النشر: | Springer Science and Business Media LLC, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Cyanobacteria, Chromatography, biology, Galdieria sulphuraria, Chemistry, General Chemical Engineering, macromolecular substances, General Chemistry, Red algae, biology.organism_classification, Mass spectrometry, Ammonium sulfate fractionation, Absorbance, Biochemistry, Phycocyanin, β subunit |
| الوصف: | Phycocyanin is a highly valuable pigmented protein synthesized by several species of cyanobacteria and red alga. In this study we demonstrate the production of thermostable phycocyanin from the unicellular red alga Galdieria sulphuraria. Phycocyanin was extracted by repeated freeze-thaw cycles and purified in a two-step process using ammonium sulfate fractionation, at 25% and 50% concentrations. Purified phycocyanin exhibited maximum absorbance at 620 nm, and the purity ratio (A620/A280) was found to be greater than 4. The recovery efficiency of phycocyanin from the crude extract was above 80%. In total, approximately 19 milligram pure phycocyanin was obtained from 3 g of wet cell mass of Galdieria sp. Subunits α and β of the protein were separated by SDS-PAGE and analyzed by MALDITOF mass spectrometry for identification, which confirmed that the isolated protein is phycocyanin. The molecular weight of α and β subunits of phycocyanin was found to be 17.6 and 18.4 kDa, respectively. |
| تدمد: | 1975-7220 0256-1115 |
| DOI: | 10.1007/s11814-013-0239-9 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::5ed69aa6197ee542005d3e3445a0c6a8 https://doi.org/10.1007/s11814-013-0239-9 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........5ed69aa6197ee542005d3e3445a0c6a8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19757220 02561115 |
|---|---|
| DOI: | 10.1007/s11814-013-0239-9 |