التفاصيل البيبلوغرافية
| العنوان: |
Immune modulation with primed mesenchymal stem cells delivered via biodegradable scaffold to repair an Achilles tendon segmental defect |
| المؤلفون: |
Sarah Duenwald-Kuehl, Erin E. Saether, Ray Vanderby, Anna E. B. Clements, Erdem Aktaş, Michael Stitgen, William L. Murphy, Jae Sung Lee, Connie S. Chamberlain, Jaclyn Kondratko-Mittnacht |
| المصدر: |
Journal of Orthopaedic Research. 35:269-280 |
| بيانات النشر: |
Wiley, 2016. |
| سنة النشر: |
2016 |
| مصطلحات موضوعية: |
0301 basic medicine, Scaffold, Achilles tendon, medicine.medical_specialty, business.industry, medicine.medical_treatment, Mesenchymal stem cell, Macrophage polarization, Bone healing, Tendon, Proinflammatory cytokine, Surgery, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, Cytokine, medicine, Cancer research, Orthopedics and Sports Medicine, business, 030217 neurology & neurosurgery |
| الوصف: |
Tendon healing is a complex coordinated series of events resulting in protracted recovery, limited regeneration, and scar formation. Mesenchymal stem cell (MSC) therapy has shown promise as a new technology to enhance soft tissue and bone healing. A challenge with MSC therapy involves the ability to consistently control the inflammatory response and subsequent healing. Previous studies suggest that preconditioning MSCs with inflammatory cytokines, such as IFN-γ, TNF-α, and IL-1β may accelerate cutaneous wound closure. The objective of this study was to therefore elucidate these effects in tendon. That is, the in vivo healing effects of TNF-α primed MSCs were studied using a rat Achilles segmental defect model. Rat Achilles tendons were subjected to a unilateral 3 mm segmental defect and repaired with either a PLG scaffold alone, MSC-seeded PLG scaffold, or TNF-α-primed MSC-seeded PLG scaffold. Achilles tendons were analyzed at 2 and 4 weeks post-injury. In vivo, MSCs, regardless of priming, increased IL-10 production and reduced the inflammatory factor, IL-1α. Primed MSCs reduced IL-12 production and the number of M1 macrophages, as well as increased the percent of M2 macrophages, and synthesis of the anti-inflammatory factor IL-4. Primed MSC treatment also increased the concentration of type I procollagen in the healing tissue and increased failure stress of the tendon 4 weeks post-injury. Taken together delivery of TNF-α primed MSCs via 3D PLG scaffold modulated macrophage polarization and cytokine production to further accentuate the more regenerative MSC-induced healing response. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:269-280, 2017. |
| تدمد: |
0736-0266 |
| DOI: |
10.1002/jor.23258 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::5d12ccafed8b147ef9a971dd03643179
https://doi.org/10.1002/jor.23258 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi...........5d12ccafed8b147ef9a971dd03643179 |
| قاعدة البيانات: |
OpenAIRE |