Polyamine Metabolism Is Altered in Unpollinated Parthenocarpicpat-2 Tomato Ovaries
| العنوان: | Polyamine Metabolism Is Altered in Unpollinated Parthenocarpicpat-2 Tomato Ovaries |
|---|---|
| المؤلفون: | Karina Proaño, Fernando Nuez, Mariano Fos, Juan Carbonell, David Alabadí, José L. García-Martínez |
| المصدر: | Plant Physiology. 131:359-366 |
| بيانات النشر: | Oxford University Press (OUP), 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | biology, Physiology, Spermine, Plant Science, Parthenocarpy, Molecular biology, Ornithine decarboxylase, Spermidine, chemistry.chemical_compound, Biochemistry, chemistry, Genetics, biology.protein, Putrescine, Spermidine synthase, Polyamine, Arginine decarboxylase |
| الوصف: | Facultative parthenocarpy induced by the recessive mutationpat-2 in tomato (Lycopersicon esculentum Mill.) depends on gibberellins (GAs) and is associated with changes in GA content in unpollinated ovaries. Polyamines (PAs) have also been proposed to play a role in early tomato fruit development. We therefore investigated whether PAs are able to induce parthenocarpy and whether thepat-2 mutation alters the content and metabolism of PAs in unpollinated ovaries. Application of putrescine, spermidine, and spermine to wild-type unpollinated tomato ovaries (cv Madrigal [MA/wt]) induced partial parthenocarpy. Parthenocarpic growth of MA/pat-2 (a parthenocarpic near-isogenic line to MA/wt) ovaries was negated by paclobutrazol (GA biosynthesis inhibitor), and this inhibition was counteracted by spermidine. Application of α-difluoromethyl-ornithine (-Orn) and/or α-difluoromethyl-arginine (-Arg), irreversible inhibitors of the putrescine biosynthesis enzymes Orn decarboxylase (ODC) and Arg decarboxylase, respectively, prevented growth of unpollinated MA/pat-2 ovaries. α-Difluoromethyl-Arg inhibition was counteracted by putrescine and GA3, whereas that of α-difluoromethyl-Orn was counteracted by GA3 but not by putrescine or spermidine. In unpollinated MA/pat-2 ovaries, the content of free spermine was significantly higher than in MA/wt ovaries. ODC activity was higher in pat-2 ovaries than in MA/wt. Transcript levels of genes encoding ODC and spermidine synthase were also higher in MA/pat-2. All together, these results strongly suggest that the parthenocarpic ability of pat-2 mutants depends on elevated PAs levels in unpollinated mutant ovaries, which correlate with an activation of the ODC pathway, probably as a consequence of elevated GA content in unpollinated pat-2tomato ovaries. |
| تدمد: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.013037 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::588ea24a7368e4fb813dca9b98771767 https://doi.org/10.1104/pp.013037 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........588ea24a7368e4fb813dca9b98771767 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
|---|---|
| DOI: | 10.1104/pp.013037 |