With the growth of the planet’s population and the depletion of mineral resources, the increase in crop yields, the search for environmentally friendly technologies, the use of enzymes and siderophores of bacterial origin, or the use of bacterial strains t hat promote plant growth (PGP ) are becoming more urgent. Was found in a strain of bacteria Pantoea brenneri AS3 produced of siderophore (82.05 μM) accounted for 28 hour culture. The strain Pantoea brenneri AS3 demonstrate antagonistic activity against all studied phytopathogenic fungi. Antagonistic activity was measured on the basis of growth inhibition of micromycetes colony compared to the control plates. The highest antagonistic activities of both strains were observed against F. solani (87%).