Objective: This work aimed to validate a simple UV-Visible spectrophotometric method for estimating Cefixime trihydrate in bulk and to produce an accurate, precise, repeatable, and cost-effective method. Material and Methods: The pH 7.4 Phosphate buffer was utilized as the solvent throughout the experiment. The drug’s absorption maxima (max) were discovered to be at 288 nm. Beer’s law was found to be obeyed in the range of 10-45 μg/mL during the quantitative analysis of the substance at 288 nm. In the doses tested, the approach was found to be linear, with the line equation y = 0.035x – 0.002 and a correlation coefficient of 0.999. Results and Discussion: Cefixime Trihydrate recovery values varied from 99.656 percent to 101.825 percent. Six duplicates of the experiment had a relative standard deviation of less than 2%. The interday precision range was 0.52-1.02%, and the intraday precision range was 0.57-0.995 percent relative standard deviation (RSD percent). The detection and quantification limits were 0.914 and 3.142 μg/mL, respectively. The method’s robustness and ruggedness had a percent relative standard deviation of 0.532-0.827 percent. Conclusion: As a result, the proposed procedure was precise, accurate, and economical. This method could be used to determine the quantity of medicine in bulk.