A recombinant flagellin fragment, which includes the epitopes flg22 and flgII-28, provides a useful tool to study flagellin-triggered immunity
| العنوان: | A recombinant flagellin fragment, which includes the epitopes flg22 and flgII-28, provides a useful tool to study flagellin-triggered immunity |
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| المؤلفون: | Serena Ciarroni, Boris A. Vinatzer, Giorgio Mariano Balestra, Haijie Liu, Christopher R. Clarke, Noam Eckshtain-Levi, Angelo Mazzaglia |
| المصدر: | Journal of General Plant Pathology. 84:169-175 |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Cloning, Genetics, Innate immune system, biology, Plant Science, Flagellum, biology.organism_classification, 01 natural sciences, Epitope, law.invention, 03 medical and health sciences, 030104 developmental biology, law, biology.protein, Pseudomonas syringae, Recombinant DNA, bacteria, Arabidopsis thaliana, Agronomy and Crop Science, Flagellin, 010606 plant biology & botany |
| الوصف: | Plants and animals independently evolved the ability to recognize flagellin (also called FliC), the building block of the bacterial flagellum, as part of their innate immune response. While animals recognize a relatively large region of FliC, most plants recognize one or two short epitopes of FliC: flg22 and flgII-28. However, since most research in plants has focused on flg22 and flgII-28 and not the actual FliC protein, the importance of any FliC region beyond the two epitopes in plant immunity is poorly understood. Here we report cloning, overexpression, and purification of a Pseudomonas syringae FliC fragment from amino acid 1 to 143, which includes both FliC epitopes and the adjacent alpha helices. Exposing Arabidopsis thaliana leaves to FliC1–143 did not reveal any additional FliC recognition capabilities beyond flg22. However, while the kiwifruit species Actinidia arguta did not respond to either flg22 or flgII-28, treatment of A. arguta leaves with FliC1–143 triggered a significant reactive oxygen response, indicating recognition. This result suggests that in some plant species, recognition of FliC requires regions of FliC beyond the two well-known epitopes and that FliC1–143 represents a useful tool in the study of plant immunity. |
| تدمد: | 1610-739X 1345-2630 |
| DOI: | 10.1007/s10327-018-0779-2 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::4af68fa2df721f1628abd29512528c9c https://doi.org/10.1007/s10327-018-0779-2 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........4af68fa2df721f1628abd29512528c9c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1610739X 13452630 |
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| DOI: | 10.1007/s10327-018-0779-2 |