An NB-LRR protein required for HR signalling mediated by both extra- and intracellular resistance proteins
| العنوان: | An NB-LRR protein required for HR signalling mediated by both extra- and intracellular resistance proteins |
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| المؤلفون: | Jack H. Vossen, S.H.E.J. Gabriëls, Daphne Y. Rainey, Frank L. W. Takken, Ahmed Abd-El-Haliem, Gerben van Ooijen, Sophia K. Ekengren, Matthieu H. A. J. Joosten, Grardy C. M. van den Berg, Pierre J. G. M. de Wit, Gregory B. Martin |
| المصدر: | The Plant Journal. 50:14-28 |
| بيانات النشر: | Wiley, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Hypersensitive response, Regulation of gene expression, MAPK/ERK pathway, Genetics, Mutation, fungi, food and beverages, Nicotiana benthamiana, Cell Biology, Plant Science, Biology, medicine.disease_cause, biology.organism_classification, Cell biology, Mutant protein, medicine, Gene silencing, Gene |
| الوصف: | Tomato (Solanum lycopersicum) Cf resistance genes confer hypersensitive response (HR)-associated resistance to strains of the pathogenic fungus Cladosporium fulvum that express the matching avirulence (Avr) gene. Previously, we identified an Avr4-responsive tomato (ART) gene that is required for Cf-4/Avr4-induced HR in Nicotiana benthamiana as demonstrated by virus-induced gene silencing (VIGS). The gene encodes a CC-NB-LRR type resistance (R) protein analogue that we have designated NRC1 (NB-LRR protein required for HR-associated cell death 1). Here we describe that knock-down of NRC1 in tomato not only affects the Cf-4/Avr4-induced HR but also compromises Cf-4-mediated resistance to C. fulvum. In addition, VIGS using NRC1 in N. benthamiana revealed that this protein is also required for the HR induced by the R proteins Cf-9, LeEix, Pto, Rx and Mi. Transient expression of NRC1(D481V), which encodes a constitutively active NRC1 mutant protein, triggers an elicitor-independent HR. Subsequently, we transiently expressed this auto-activating protein in N. benthamiana silenced for genes known to be involved in HR signalling, thereby allowing NRC1 to be positioned in an HR signalling pathway. We found that NRC1 requires RAR1 and SGT1 to be functional, whereas it does not require NDR1 and EDS1. As the Cf-4 protein requires EDS1 for its function, we hypothesize that NRC1 functions downstream of EDS1. We also found that NRC1 acts upstream of a MAP kinase pathway. We conclude that Cf-mediated resistance signalling requires a downstream NB-LRR protein that also functions in cell death signalling pathways triggered by other R proteins. |
| تدمد: | 1365-313X 0960-7412 |
| DOI: | 10.1111/j.1365-313x.2007.03027.x |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::490c167cff915ee480a8e8d082b2abfb https://doi.org/10.1111/j.1365-313x.2007.03027.x |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........490c167cff915ee480a8e8d082b2abfb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1365313X 09607412 |
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| DOI: | 10.1111/j.1365-313x.2007.03027.x |