In vitro studies on Pea (Pisum sativum L.): I. Callus formation, regeneration and rooting

التفاصيل البيبلوغرافية
العنوان: In vitro studies on Pea (Pisum sativum L.): I. Callus formation, regeneration and rooting
المؤلفون: Abdelfattah Badr, S. A. Ghanem, M. K. El-Bahr, M. M. Saker
المصدر: Giornale botanico italiano. 130:695-705
بيانات النشر: Informa UK Limited, 1996.
سنة النشر: 1996
مصطلحات موضوعية: food.ingredient, Callus formation, fungi, food and beverages, Organogenesis, Plant Science, Biology, Hypocotyl, Murashige and Skoog medium, food, Callus, Shoot, Botany, Ecology, Evolution, Behavior and Systematics, Cotyledon, Explant culture
الوصف: Callus production, shoot formation via organogenesis and rooting of the regenerated shoots are reported in an Egyptian variety of Pisum sativum L. Calli were initiated from hypocotyl, leaf, root and mature embryo explants when cultured on MS medium containing B5 vitamins and supplemented with 2 mg/l 2,4-D+1 mg/l kin. Among the different types of explants, hypocotyl showed best potential for callus proliferation. Hypocotyl, leaf and immature cotyledon explants were used for shoot organogenesis. The best results of shoot formation were achieved when hypocotyl explants were cultured on MS-medium supplemented with 2 mg/l BA+1 mg/l NAA. However, immature cotyledon explants showed the highest frequency of shoot formation with 1 mg/l BA. Data of in vitro rooting showed that maximum root frequency occurred on culture medium containing half strength of MS salts, 40 g/l sucrose and 2 mg/l NAA.
تدمد: 0017-0070
DOI: 10.1080/11263509609438342
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::3eaa02b9aa752b9179de2d2594f8fc3d
https://doi.org/10.1080/11263509609438342
رقم الانضمام: edsair.doi...........3eaa02b9aa752b9179de2d2594f8fc3d
قاعدة البيانات: OpenAIRE
الوصف
تدمد:00170070
DOI:10.1080/11263509609438342