Increased litter input significantly changed the total and active microbial communities in degraded grassland soils
| العنوان: | Increased litter input significantly changed the total and active microbial communities in degraded grassland soils |
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| المؤلفون: | Linfeng Li, Zhihong Xu, Xiaoyong Cui, Jinming Hu, Jinling Qin, Weijin Wang, Rongxiao Che, Dong Liu, Fang Wang, Iman Tahmasbian |
| المصدر: | Journal of Soils and Sediments. 20:2804-2816 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Denitrification, Stratigraphy, Phosphorus, Grassland degradation, chemistry.chemical_element, 04 agricultural and veterinary sciences, 010501 environmental sciences, 01 natural sciences, chemistry, Agronomy, Microbial population biology, 040103 agronomy & agriculture, Litter, 0401 agriculture, forestry, and fisheries, Organic matter, Microcosm, Relative species abundance, 0105 earth and related environmental sciences, Earth-Surface Processes |
| الوصف: | Increasing organic matter input and phosphorus fertilization are employed extensively to restore degraded grasslands. Nevertheless, little is known about their effects on microbes, especially on active microbial populations. Therefore, this study is aimed at examining the short-term influences of litter and phosphorus addition on microbes in degraded grassland soils. A microcosm experiment was established using soils sampled from a heavily degraded Tibetan alpine meadow. The experiment used a two-way factorial design with grass litter and phosphorus addition as the main factors. Microbial abundance and rDNA transcriptional activity were assessed through quantitative PCR. Total and active microbial community profiles were measured using DNA- and RNA-based MiSeq sequencing, respectively. As shown in this study, litter addition significantly increased microbial rDNA transcriptional activity and fungal abundance, but it decreased microbial α-diversity. However, prokaryote abundance was unaffected by the litter addition. Total and active soil microbial community profiles and interaction patterns were also significantly altered by litter addition. The relative abundance of copiotrophic and oligotrophic microbial lineages significantly increased and decreased, respectively, in the soils with litter addition. Functional predictions suggested that litter addition might significantly increase the abundance of pathogens, as well as microbes related to nitrogen fixation, denitrification, and chitinolysis, while decreasing nitrifier abundance. In contrast, no significant effects of the phosphorus addition on soil microbes were observed. These findings highlight the significant effects of increasing litter input on total and active soil microbial communities and suggest that microbial responses should be considered when restoring degraded grasslands by increasing organic matter input. |
| تدمد: | 1614-7480 1439-0108 |
| DOI: | 10.1007/s11368-020-02619-x |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::3d3d8866d306c9c9e44ee52a13377af5 https://doi.org/10.1007/s11368-020-02619-x |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........3d3d8866d306c9c9e44ee52a13377af5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16147480 14390108 |
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| DOI: | 10.1007/s11368-020-02619-x |