Development and Study of a Method for Cell Separation During White Blood Cell Segmentation on Images of Bone Marrow Preparations in Information and Measurement Systems for Diagnostics of Acute Leukemia
| العنوان: | Development and Study of a Method for Cell Separation During White Blood Cell Segmentation on Images of Bone Marrow Preparations in Information and Measurement Systems for Diagnostics of Acute Leukemia |
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| المؤلفون: | V. V. Dmitrieva, V. Yu. Sel’chuk, A. N. Pronichev, E. V. Polyakov, V. G. Nikitaev, A. D. Samsonova, N. N. Tupitsyn |
| المصدر: | Measurement Techniques. 63:587-590 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Acute leukemia, Computer science, Applied Mathematics, 010401 analytical chemistry, High density, 01 natural sciences, 0104 chemical sciences, 010309 optics, Digital image, medicine.anatomical_structure, Feature (computer vision), White blood cell, 0103 physical sciences, Cell separation, medicine, Segmentation, Bone marrow, Instrumentation, Biomedical engineering |
| الوصف: | The issues of using information and measurement systems based on processing of digital images of microscopic preparations for solving large-scale tasks of automating the diagnosis of acute leukemia are considered. The high density of leukocyte cells in the preparation (hypercellularity) is a feature of microscopic images of bone marrow preparations. It causes the proximity of cells to each other and their contact with the formation of conglomerates. Measuring of the characteristics of bone marrow cells in such conditions leads to unacceptable errors (over 50%). The work is devoted to segmentation of contiguous cells in images of bone marrow preparations. A method of cell separation during white blood cell segmentation on images of bone marrow preparations in conditions of hypercellularity of the preparation has been developed. The peculiarity of the proposed method is the use of an approach to segmentation of cell images based on the watershed method with markers. Key stages of the method: the formation of initial markers and building of the watershed lines, threshold binarization, shading inside the outline. The parameters of the separation of contiguous cells are determined. The experiment confirmed the effectiveness of the proposed method. The relative segmentation error was 5 %. The use of the proposed method in information and measurement systems of computer microscopy for automated analysis of bone marrow preparations will help to improve the accuracy of diagnosis of acute leukemia. |
| تدمد: | 1573-8906 0543-1972 |
| DOI: | 10.1007/s11018-020-01816-x |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::3aa3d34f52d3be9d1f69e8f3f28329ea https://doi.org/10.1007/s11018-020-01816-x |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........3aa3d34f52d3be9d1f69e8f3f28329ea |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15738906 05431972 |
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| DOI: | 10.1007/s11018-020-01816-x |