Comparison of slow freezing and vitrification methods for Venda cockerel’s spermatozoa
| العنوان: | Comparison of slow freezing and vitrification methods for Venda cockerel’s spermatozoa |
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| المؤلفون: | M. L. Mphaphathi, Dibungi Luseba, Ben Sutherland, Tshimangadzo Lucky Nedambale |
| المصدر: | Open Journal of Animal Sciences. :204-210 |
| بيانات النشر: | Scientific Research Publishing, Inc., 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | endocrine system, urogenital system, Extender, Semen, Straw, Biology, Semen cryopreservation, Sperm, Cryopreservation, law.invention, Andrology, law, Vitrification, Sperm motility |
| الوصف: | An improvement in avian semen cryopreservation is essential and has the potential to improve the cryo-gene banking efficiency. This study compared two cryopreservation methods (slow freezing and vitrification) and the effect of different thawing/warming temperatures (5℃, 25℃ and 41℃) on Venda cockerel’s spermatozoa. Semen samples from Venda cockerels were diluted with modified Kobidil+ extender supplemented with 8% dimethyl sulfoxide. Semen from each ejaculate was stained with nigrosin/eosin for viability examination. The cryopreserved samples were either slow cooled in 0.25 mL straw or vitrified in a solid surface vitrification (SSV) device. Semen straw or cryovial was stored in liquid nitrogen container. The straw or cryovial with sperm was thawed or warmed at 5?C, 25?C and 41℃ and analysed by a Computer-Aided Sperm Analysis (CASA). There was a significant difference in live/normal sperm between the semen donors. Cockerels spermatozoa cryopreserved by slow freezing (43%) and thawed at 5?C had a significantly higher survival and motility rate compared to vitrification (2.5%) method. In conclusion, there was higher rate of live/normal morphology sperm. Cryopreservation process reduces sperm motility and velocity rate regardless of cryoprevervation method and thawing or warming temperatures. However, slow freezing was a better method to maintain motility of spermatozoa following cryopreservation. |
| تدمد: | 2161-7627 2161-7597 |
| DOI: | 10.4236/ojas.2012.23028 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::397e78b093da5f0109167e9a89a80581 https://doi.org/10.4236/ojas.2012.23028 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........397e78b093da5f0109167e9a89a80581 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21617627 21617597 |
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| DOI: | 10.4236/ojas.2012.23028 |