Application of the TaqMan®real-time PCR assay for the detection of chicken (Gallus gallus) meat in pork products

التفاصيل البيبلوغرافية
العنوان: Application of the TaqMan®real-time PCR assay for the detection of chicken (Gallus gallus) meat in pork products
المؤلفون: Seong-Do Park, Ji-Yeon Kim, Yong-Hwan Kim, Ho-Myung Na, Ba-Ra-Da Koh
المصدر: Korean Journal of Veterinary Service. 36:193-201
بيانات النشر: The Korean Society of Veterinary Service, 2013.
سنة النشر: 2013
مصطلحات موضوعية: Veterinary medicine, Real-time polymerase chain reaction, Labelling, Pcr assay, TaqMan, Food labelling, food and beverages, Food science, Biology
الوصف: Many consumers are increasingly concerned about the meat they eat, and accurate labelling is important due to public health, economic and legal concerns. Meat species adulteration is a common problem in the retail markets. In this study, a TaqMan Ⓡ quantitative real-time polymerase chain reaction (PCR) as-say was applied for its ability to quantify chicken meat, which was not indicated on the label, in 79 commercial pork products (ham, sausages, bacon and ground meat) producted by 10 different manufacturers. The amplification efficiency was 82.05% and the square regression coefficient (R 2 ) was 0.995. PCR results showed that 38.6% of ham samples, 50.0% of sausages samples, and 50.0% of ground meat samples were contaminated with chicken residuals, while the bacon samples were not con-taminated with chicken residuals. Only twelve pork products of one of the manufacturers were in ac-cordance with indicated in their labels. The PCR assay reported in this work could be particularly useful in inspection programs to verify the food labelling of commercial processed meats and to gain consum-ers' trust.
تدمد: 1225-6552
DOI: 10.7853/kjvs.2013.36.3.193
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::35223d63272c02c55187d7fe965fd3a0
https://doi.org/10.7853/kjvs.2013.36.3.193
Rights: OPEN
رقم الانضمام: edsair.doi...........35223d63272c02c55187d7fe965fd3a0
قاعدة البيانات: OpenAIRE
الوصف
تدمد:12256552
DOI:10.7853/kjvs.2013.36.3.193