Improved salt tolerance of transgenic wheat by introducing betA gene for glycine betaine synthesis
| العنوان: | Improved salt tolerance of transgenic wheat by introducing betA gene for glycine betaine synthesis |
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| المؤلفون: | Weiwei Zhang, Qiang Gao, Chunmei He, Juren Zhang, Aifang Yang |
| المصدر: | Plant Cell, Tissue and Organ Culture (PCTOC). 101:65-78 |
| بيانات النشر: | Springer Science and Business Media LLC, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Choline dehydrogenase, Transgene, food and beverages, Plant physiology, Genetically modified crops, Horticulture, Biology, chemistry.chemical_compound, Transformation (genetics), Betaine, chemistry, Biochemistry, Chlorophyll, Botany, Halotolerance |
| الوصف: | A betA gene encoding choline dehydrogenase from Escherichia coli (E. coli) was transformed into wheat (Triticum aestivum L.) via Agrobacterium-mediated transformation. PCR amplification and Southern blotting confirmed the existence of transgene in transformed plants and their progeny. Levels of expression of the betA gene varied among the different transgenic lines based on RT-PCR analysis. Under salt stress conditions, transgenic lines L2 and L3 had higher levels of glycine betaine and chlorophyll, lower Na+/K+ ratios and solute potential, and less cell membrane damage. These lines also retained moderately higher photosynthesis rates and more vigorous growth than the wild-type line at 200 mM NaCl. In a field trial in a high salt field, transgenic lines L2 and L3 had higher germination rates, more tillers and higher grain yields in comparison to the wild-type plants. This suggested that the transgenic plants were more tolerant to salt stress and have potential for breeding salt-tolerant wheat. |
| تدمد: | 1573-5044 0167-6857 |
| DOI: | 10.1007/s11240-009-9665-0 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::34c15ffb95b828531af21b10773fae96 https://doi.org/10.1007/s11240-009-9665-0 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........34c15ffb95b828531af21b10773fae96 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15735044 01676857 |
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| DOI: | 10.1007/s11240-009-9665-0 |