Paracoccus simplex sp. nov., a New Methylamine-Utilizing Facultative Methylotroph
| العنوان: | Paracoccus simplex sp. nov., a New Methylamine-Utilizing Facultative Methylotroph |
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| المؤلفون: | Nina V. Doronina, Yu. A. Trotsenko, A. A. Chemodurova, E. N. Kaparullina |
| المصدر: | Microbiology. 87:662-671 |
| بيانات النشر: | Pleiades Publishing Ltd, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Oxidase test, Strain (chemistry), biology, Chemistry, Methylamine, Hydroxypyruvate reductase, 030106 microbiology, Amine dehydrogenase, biology.organism_classification, Applied Microbiology and Biotechnology, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, Paracoccus, Biochemistry, biology.protein, Methylotroph, Energy source |
| الوصف: | A facultative methylotroph, strain F5Т, which uses methylamine and a broad spectrum of polycarbon substrates as carbon and energy sources, was isolated from silt of a freshwater lake in the southern Moscow region. The cells were gram-negative, coccoid, non-spore-forming, nonmotile, colorless, reproducing by binary fission and possessing a capsule. The organism was mesophilic, neutrophilic, not halophilic, oxidase- and catalase-positive, and capable of nitrate reduction to nitrite. Methylamine was oxidized by amine dehydrogenase and via the icl- serine pathway of С1 metabolism, as was indicated by activities of hydroxypyruvate reductase and serine-glyoxylate aminotransferase and by the absence of hexulosephosphate synthase and ribulose bisphosphate carboxylase. Predominant fatty acids were C18:1ω7c (72.3%) and C16:0 (11.6%). Phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylethanolamine were the dominant phospholipids. The G + C DNA content was 65.8 mol % (Tm). Q10 was the dominant ubiquinone. Strain F5T exhibited high similarity of the 16S rRNA gene sequences with Paracoccus strains: P. aminovorans JCM7685T = VKM B-2140Т (98.0%), P. huijuniae FLN-7Т (97.9%), and P. limosus NB88T (97.5%). However, the level of DNA-DNA relatedness between the strains F5Т and P. aminovoransТ was only 21 ± 3%. Based on the data obtained, strain F5Т was identified as a new Paracoccus species with proposed name Paracoccus simplex sp. nov. (= VKM B-3226T = CCUG 71989T). |
| تدمد: | 1608-3237 0026-2617 |
| DOI: | 10.1134/s0026261718050077 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::2f6c40ffe70b50ddec4ad431b3a0d332 https://doi.org/10.1134/s0026261718050077 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........2f6c40ffe70b50ddec4ad431b3a0d332 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16083237 00262617 |
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| DOI: | 10.1134/s0026261718050077 |