Development of Robust SCAR Markers that Distinguish the Six Cultivated Brassica Species and Subspecies of the U-triangle
| العنوان: | Development of Robust SCAR Markers that Distinguish the Six Cultivated Brassica Species and Subspecies of the U-triangle |
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| المؤلفون: | Michell E. Sass, Geunhwa Jung, Mitrick A. Johns, Federico L. Iñiguez-Luy, James Nienhuis |
| المصدر: | Journal of the American Society for Horticultural Science. 131:424-432 |
| بيانات النشر: | American Society for Horticultural Science, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Germplasm, Genetic marker, Genetic variation, Botany, Genetics, Brassica, Amplified fragment length polymorphism, Horticulture, Restriction fragment length polymorphism, Biology, Subspecies, biology.organism_classification, RAPD |
| الوصف: | This research describes the development of robust molecular descriptors that are reliable and easy to use (PCR-based) for the proper classification of the six cultivated Brassica L. species and subspecies that make up the U triangle. Sequence characterized amplified regions (SCAR) were derived from DNA fragments generated by randomly amplified polymorphic DNA (RAPD) primers that distinguished the U-triangle Brassica species: B. rapa L. (A, n = 10), B. nigra (L.) Koch (B, n = 8), B. oleracea L. (C, n = 9), B. juncea (L.) Czern (AB, n = 18), B. napus L. (AC, n = 19), and B. carinata Braun (BC, n = 17). The SCAR descriptors developed in this study reveal the presence-absence of a DNA fragment and sequence length polymorphisms to distinguish the three genomes (A, B, and C) of the six cultivated Brassica species. These markers should prove to be useful in revealing misclassifications at the subspecies level. The amplification of a single DNA fragment and reduced sensitivity to reaction conditions make these SCAR descriptors ideal to accurately classify and organize large numbers of cultivated Brassica accessions typically present in large germplasm collections. |
| تدمد: | 2327-9788 0003-1062 |
| DOI: | 10.21273/jashs.131.3.424 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::2e85f74f2595f9b8b185de0aa44d7712 https://doi.org/10.21273/jashs.131.3.424 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........2e85f74f2595f9b8b185de0aa44d7712 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23279788 00031062 |
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| DOI: | 10.21273/jashs.131.3.424 |