High-Content Analysis Monitoring Intracellular Trafficking and Replication of Mycobacterium tuberculosis Inside Host Cells

التفاصيل البيبلوغرافية
العنوان: High-Content Analysis Monitoring Intracellular Trafficking and Replication of Mycobacterium tuberculosis Inside Host Cells
المؤلفون: Arnaud Machelart, Eik Hoffmann, Alexandre Vandeputte, Imène Belhaouane, Nathalie Deboosere, Priscille Brodin
المصدر: Methods in Molecular Biology ISBN: 9781071614594
بيانات النشر: Springer US, 2021.
سنة النشر: 2021
مصطلحات موضوعية: Tuberculosis, Heterologous, Bacillus, Biology, biology.organism_classification, medicine.disease, law.invention, Cell biology, Mycobacterium tuberculosis, Confocal microscopy, law, High-content screening, medicine, Intracellular, Bacteria
الوصف: Mycobacterium tuberculosis is able to colonize, persist, and massively replicate in host cells, such as phagocytes and epithelial cells. The intracellular stage of the bacteria is critical to the development of tuberculosis pathogenesis. The detailed mechanisms of intracellular trafficking of the bacillus are not fully understood and require further investigations. Therefore, increasing the knowledge of this process will help to develop therapeutic tools that will lower the burden of tuberculosis. M. tuberculosis is genetically tractable and tolerates the expression of heterologous fluorescent proteins. Thus, the intracellular distribution of the bacteria expressing fluorescent tracers can be easily defined using confocal microscopy. Advances in imaging techniques and images-based analysis allow the rapid quantification of biological objects in complex environments. In this chapter, we detailed high-content / high-throughput imaging methods to track the bacillus within host cell settings.
ردمك: 978-1-07-161459-4
DOI: 10.1007/978-1-0716-1460-0_29
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::2b0564df119dc3a1fdde1ba2a647ef59
https://doi.org/10.1007/978-1-0716-1460-0_29
Rights: CLOSED
رقم الانضمام: edsair.doi...........2b0564df119dc3a1fdde1ba2a647ef59
قاعدة البيانات: OpenAIRE
الوصف
ردمك:9781071614594
DOI:10.1007/978-1-0716-1460-0_29