Abstract 81: Studying Ghanian Cancer Genomes Using Cell-free DNA
| العنوان: | Abstract 81: Studying Ghanian Cancer Genomes Using Cell-free DNA |
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| المؤلفون: | Kevin Gardner, Carlos Caldas, Xiaoyu Song, Daniel Ansong, Verne Vanderpuye, Francis Aitpillah, Wiafe-Addai, Ernest Adjei, Joel Yarney, Kofi Mensah Nyarko, Nicholas Titiloye, Montserrat Garcia-Closas, Gavin Ha, Mustapha Abubakar, Anna-Lisa Doebley, William D. Foulkes, Paz Polak, Baffour Awuah, Joe-Nat Clegg-Lamptey, Alexander Kwarteng, John M S Bartlet, Thomas U. Ahearn, Máire A. Duggan, Samuel Terkper Ahuno, Anne M. Bowcock, Nancy Hamel, Lawrence Edusei, Jonine D. Figueroa, Daniel G. Stover, Seth Wiafe |
| المصدر: | Cancer Epidemiology, Biomarkers & Prevention. 30:81-81 |
| بيانات النشر: | American Association for Cancer Research (AACR), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Copy number gain, Genetics, Breast cancer, Oncology, Cell-free fetal DNA, Epidemiology, Concordance, medicine, Cancer, Biology, medicine.disease, Genome, Cancer Etiology |
| الوصف: | Purpose: Analysis of cell free DNA could provide a rapid and non-invasive approach to detect cancer and provide new molecular insights in many African countries where expert pathology is lacking. Hence, we tested whether whole-genome sequencing of cfDNA (WGS-cfDNA) could identify somatic alterations that drive breast cancer. Methods: We conducted a pilot on 15 Ghanaian women (median age 49.5 years) recruited as part of the Ghana Breast Health Study. cfDNA was extracted and subjected to WGS at 30x and 0.1x. ichorCNA software was used to predict copy number alterations and ctDNA fractions. Results: We found extensive amplification and deletion of multiple chromosomal regions including those with oncogenes and tumor suppressor genes associated with breast cancer. Similar copy number alterations for selected breast cancer genes were observed with 0.1x and 30x cfDNA-WGS with increasing concordance between the two instruments as the ctDNA fraction increases. We observed a high frequency (>50%) of copy number gain in 3/5 regions and potential target genes for the amplification (chr8p11-12 [ZNF703] n=8, 53.3%; chr8q24.2 [MYC] n=9, 60%; chr19q12 [CCNE1] n=9, 60%), which were in agreement to previous observations among African-American (AA) ancestry compared to European-American (EA) ancestry in TCGA datasets. Conclusion: Our data provided evidence that ctDNA-based genomic studies are possible and ctDNA analysis could be a tool for future molecular oncology studies in Africa for cancer etiology, surveillance and clinical trials. Citation Format: Samuel Ahuno, Anna-Lisa Doebley, Thomas Ahearn, Joel Yarney, Nicholas Titiloye, Nancy Hamel, Ernest Adjei, Joe-Nat Clegg-Lamptey, Lawrence Edusei, Baffour Awuah, Xiaoyu Song, Verne Vanderpuye, Mustapha Abubakar, Maire Duggan, Daniel Stover, Kofi Nyarko, John Bartlet, Francis Aitpillah, Daniel Ansong, Kevin Gardner, Anne Bowcock, Carlos Caldas, William Foulkes, Seth Wiafe, Wiafe-Addai, Montserrat Garcia-Closas, Alexander Kwarteng, Gavin Ha, Jonine Figueroa, Paz Polak, On Behalf Of Ghana Breast Health Study Team. Studying Ghanian Cancer Genomes Using Cell-free DNA [abstract]. In: Proceedings of the 9th Annual Symposium on Global Cancer Research; Global Cancer Research and Control: Looking Back and Charting a Path Forward; 2021 Mar 10-11. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2021;30(7 Suppl):Abstract nr 81. |
| تدمد: | 1538-7755 1055-9965 |
| DOI: | 10.1158/1538-7755.asgcr21-81 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::2a78f3414b1dc82176521b13e3173465 https://doi.org/10.1158/1538-7755.asgcr21-81 |
| رقم الانضمام: | edsair.doi...........2a78f3414b1dc82176521b13e3173465 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15387755 10559965 |
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| DOI: | 10.1158/1538-7755.asgcr21-81 |