This chapter examines four case studies of conservation sites and nine green roofs located in the Shortgrass Prairie and Rocky Mountain ecoregions. The shortgrass prairie once covered the Great Plains from the Texas Panhandle in the south to Alberta, Canada, in the north, and from western Kansas in the east to Montana in the west. With 300–400 mm of annual precipitation, the shortgrass prairie vegetation is much shorter in stature than the mixed-grass or tallgrass prairies, however, it is ecologically significant. Topographic relief has a dramatic effect on plant diversity, as the natural vegetation of open plains tend to be simple compositions where drainages and transitions to dry habitats can be highly diverse. The Rocky Mountains form a massive range with many ecoregions including mountain meadows and alpine plant communities. Ecoregional green roofs located in Laramie, Wyoming, Boulder, Colorado, Denver, Colorado, and the Rocky Mountains represent 347 taxa in total, 274 of which are native to the ecoregions in this chapter.