التفاصيل البيبلوغرافية
| العنوان: |
Additional file 7 of Characterization of surface markers on extracellular vesicles isolated from lymphatic exudate from patients with breast cancer |
| المؤلفون: |
Ekstr��m, Karin, Crescitelli, Rossella, P��tursson, Hafsteinn Ingi, Johansson, Junko, L��sser, Cecilia, Bagge, Roger Olofsson |
| بيانات النشر: |
figshare, 2022. |
| سنة النشر: |
2022 |
| الوصف: |
Additional file 7. Uncropped western blot images. Uncropped images of all western blots included in the manuscript are shown in the figure. For patient 3, 5 and 6, proteins extracted from individual SEC fractions (F1���7 or 8) were separated on the gel and blotted for GRP94, albumin, CD63, Flotillin-1 and ApoA1. Proteins extracted from patient 1���7 was analyzed for albumin, CD63, Flotillin-1, ApoA1 and CD146. In each lane, 10 ��g of proteins (9 for patient 2) was loaded for pooled EV fraction 2���4 and 20 ��g for individual fractions except for F1 in patient 4 and 6 where the maximum volume was loaded (2.5 and 3.4 ��g respectively) due to low protein concentration in those samples. The positive control (CTR+) used was proteins extracted from MSC cell lysate (Grp94 and Flotillin-1), EVs from MSCs (CD63), human melanoma metastatic tissue (albumin and ApoA1). For CD146, the unstained gel is shown which was used for total protein normalization step. |
| DOI: |
10.6084/m9.figshare.18132283.v1 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::2676e3b7397c5ecad0c0014cf4c2be91 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi...........2676e3b7397c5ecad0c0014cf4c2be91 |
| قاعدة البيانات: |
OpenAIRE |