CK2 and Akt (also known as PKB) are two antiapoptotic protein kinases, which phosphorylate hundreds of substrates. While CK2 is constitutively active, Akt, under physiological conditions, is activated by external growth/proliferation signals. However, in cancer, on the one hand, the mechanism of Akt activation is frequently dysregulated and, on the other, CK2 is usually overexpressed. Both kinases are therefore involved in tumorigenesis and considered promising drug targets.