Desensitization of Muscarinic Receptors
| العنوان: | Desensitization of Muscarinic Receptors |
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| المؤلفون: | Karen Vermis, J Van Emmelo, Rh Paulssen, Norbert Fraeyman |
| المصدر: | Receptors and Channels. 10:1-10 |
| بيانات النشر: | Informa UK Limited, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Pharmacology, medicine.medical_specialty, Chinese hamster ovary cell, medicine.medical_treatment, Clinical Biochemistry, Stimulation, Cell Biology, Biology, Wortmannin, chemistry.chemical_compound, Endocrinology, chemistry, Internal medicine, Muscarinic acetylcholine receptor, medicine, Protein kinase A, Receptor, Protein kinase C, Desensitization (medicine) |
| الوصف: | When Chinese hamster ovary cells transfected with the gene for M(3)-muscarinic receptors were stimulated with carbachol continuously for 30 min, the response at the end of the stimulation period was about 20% of the early response (2-3 min after the start of the stimulation). Long-term treatment of the cells with phorbol ester abolished the response completely while desensitization was significantly reduced upon pre-treatment of the cells with GF109203X, antisense oligonucleotide against the alpha-isoform of protein kinase C and wortmannin. We conclude that in the Chinese hamster ovary expression system, desensitization of M(3)-muscarinic receptors is dependent on a fast feedback loop including the alpha-isoform of protein kinase C. |
| تدمد: | 1543-5334 1060-6823 |
| DOI: | 10.3109/10606820490281175 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::10b7f0946bfc4356d570654383956b2d https://doi.org/10.3109/10606820490281175 |
| رقم الانضمام: | edsair.doi...........10b7f0946bfc4356d570654383956b2d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15435334 10606823 |
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| DOI: | 10.3109/10606820490281175 |