Antibacterial and antioxidant potential of ethyl acetate extract from Streptomyces AIA12 and AIA17 isolated from gut of Chanos chanos
| العنوان: | Antibacterial and antioxidant potential of ethyl acetate extract from Streptomyces AIA12 and AIA17 isolated from gut of Chanos chanos |
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| المؤلفون: | Muhammad Alfid Kurnianto, Ekowati Chasanah, Hanifah Nuryani Lioe, Harsi Dewantari Kusumaningrum |
| المصدر: | Biodiversitas Journal of Biological Diversity. 22 |
| بيانات النشر: | UNS Solo, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Growth medium, Antioxidant, biology, medicine.medical_treatment, Bacillus cereus, Ethyl acetate, Plant Science, Antimicrobial, biology.organism_classification, medicine.disease_cause, Ascorbic acid, Streptomyces, chemistry.chemical_compound, chemistry, medicine, Animal Science and Zoology, Food science, Molecular Biology, Escherichia coli |
| الوصف: | Kurnianto MA, Kusumaningrum HD, Lioe HN, Chasanah E. 2021. Antibacterial and antioxidant potential of ethyl acetate extract from Streptomyces AIA12 and AIA17 isolated from gut of Chanos chanos. Biodiversitas 22: 3196-3206. Streptomyces has been recognized as a promising and productive source of antibacterial and antioxidant compounds. The phenotypic and genomic characterizations indicated AIA12 and AIA17 are closely related to the S. globisporus ARGB01 and S. misionensis S1-SC15, respectively. Incubation on yeast-malt extract broth for nine days (AIA12) and 11 days (AIA17) proved to be the best growth medium and optimum antibacterial production time for these two strains. Crude-extract of extracellular secondary metabolites, obtained by ethyl acetate extraction, demonstrated broad-spectrum inhibitory activity against Pseudomonas aeruginosa InaCC B52, Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 10876, Escherichia coli ATCC 25922, Salmonella Typhimurium ATCC 14028, and Listeria monocytogenes with minimum inhibitory and minimum bactericidal concentrations from 2.5 to 0.31 and 5.0 to 0.31 mg mL-1, respectively. Evaluation of antioxidant showed AIA17 crude-extract had moderate DPPH scavenging and antioxidant activities of 65.122% ± 0.56 and 28.178 ± 0.24 mg ascorbic acid equivalent antioxidant capacity (AEAC g-1), respectively. The identification of compounds through profiling with RP-HPLC showed optimum absorbance at 210 and 214 nm, which showed the presence of peptide groups in the constituent compounds' molecular structure. These findings indicate that Chanos chanos-derived Streptomyces produces valuable bioactive compounds with various promising biological activities. |
| تدمد: | 2085-4722 1412-033X |
| DOI: | 10.13057/biodiv/d220813 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::0e49f00333489ee0a6d4a3346eccec0c https://doi.org/10.13057/biodiv/d220813 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........0e49f00333489ee0a6d4a3346eccec0c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20854722 1412033X |
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| DOI: | 10.13057/biodiv/d220813 |