التفاصيل البيبلوغرافية
| العنوان: |
Data from Immuno-PET Imaging of Engineered Human T Cells in Tumors |
| المؤلفون: |
Angela M. Krackhardt, Calogero D'Alessandria, Markus Schwaiger, Mona Mustafa, Sibylle Ziegler, Christian Peschel, Michaela Aichler, Iina Laitinen, Stefan Audehm, Melanie Straub, Katja Steiger, Henrique Bianchi, Richard Klar, Ricarda Wagner, Nahid Yusufi, Sabine Mall |
| بيانات النشر: |
American Association for Cancer Research (AACR), 2023. |
| سنة النشر: |
2023 |
| الوصف: |
Sensitive in vivo imaging technologies applicable to the clinical setting are still lacking for adoptive T-cell–based immunotherapies, an important gap to fill if mechanisms of tumor rejection or escape are to be understood. Here, we propose a highly sensitive imaging technology to track human TCR-transgenic T cells in vivo by directly targeting the murinized constant TCR beta domain (TCRmu) with a zirconium-89 (89Zr)-labeled anti–TCRmu-F(ab')2 fragment. Binding of the labeled or unlabeled F(ab')2 fragment did not impair functionality of transgenic T cells in vitro and in vivo. Using a murine xenograft model of human myeloid sarcoma, we monitored by Immuno-PET imaging human central memory T cells (TCM), which were transgenic for a myeloid peroxidase (MPO)–specific TCR. Diverse T-cell distribution patterns were detected by PET/CT imaging, depending on the tumor size and rejection phase. Results were confirmed by IHC and semiquantitative evaluation of T-cell infiltration within the tumor corresponding to the PET/CT images. Overall, these findings offer a preclinical proof of concept for an imaging approach that is readily tractable for clinical translation. Cancer Res; 76(14); 4113–23. ©2016 AACR. |
| DOI: |
10.1158/0008-5472.c.6507479.v1 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::0c778eaca6690d1bae23be696e2ba313
https://doi.org/10.1158/0008-5472.c.6507479.v1 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi...........0c778eaca6690d1bae23be696e2ba313 |
| قاعدة البيانات: |
OpenAIRE |