Phosphate-buffered native gel electrophoresis of E. Coli H-NS
| العنوان: | Phosphate-buffered native gel electrophoresis of E. Coli H-NS |
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| المؤلفون: | Choong-Min Kang, Ji-Hwan Yook, Woo-Yeon Kim |
| المصدر: | Applied Biological Chemistry. 59:677-679 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Gel electrophoresis, biology, Sodium, 030106 microbiology, Organic Chemistry, Mutant, chemistry.chemical_element, biology.organism_classification, Molecular biology, General Biochemistry, Genetics and Molecular Biology, Histone-like nucleoid-structuring protein, 03 medical and health sciences, Histone, chemistry, biology.protein, bacteria, Nucleoid, Bioorganic chemistry, Bacteria |
| الوصف: | Histone-like nucleoid structuring protein (H-NS) is a global regulator in enteric bacteria and other bacteria. It has been reported that H-NS has no modifications for its activity. Even though H-NS is called histone-like protein, it is acidic, contrary to the basic eukaryotic histones. Here, we report that there were mobility differences for various sources of H-NS seen in sodium phosphate-buffered native gel electrophoresis. We examined various native gel electrophoresis conditions using the wild-type E. coli (MG1655), three H-NS mutants (two were H-NS knockout mutants), and the E. coli BL21 overexpressing H-NS (BL21 (DE3)/pPD3). |
| تدمد: | 2468-0842 2468-0834 |
| DOI: | 10.1007/s13765-016-0214-3 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::0afeb41e2df8909d832890c5857fae94 https://doi.org/10.1007/s13765-016-0214-3 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........0afeb41e2df8909d832890c5857fae94 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24680842 24680834 |
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| DOI: | 10.1007/s13765-016-0214-3 |