Phase separation drives the self-assembly of mitochondrial nucleoids for transcriptional modulation
| العنوان: | Phase separation drives the self-assembly of mitochondrial nucleoids for transcriptional modulation |
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| المؤلفون: | Jingyi Guo, Wei Li, Juntao Qi, Shouhua Ye, Hui Zhao, Heng Mao, Liang Wang, Yi Wu, Ge Xiang, Yonglong Chen, Jinsong Liu, Mingli Hu, Qi Long, Xingguo Liu, Pilong Li, Wai-Yee Chan, Yanshuang Zhou, Shiwei Du, Junwei Wang, Liang Yang, Hao Wu, Jiayuan Wen |
| المصدر: | Nature Structural & Molecular Biology. 28:900-908 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Mitochondrial DNA, animal structures, Chemistry, fungi, TFAM, Mitochondrion, Cell biology, Chromatin, Structural Biology, Transcription (biology), bacteria, Nucleoid, Molecular Biology, Mitochondrial nucleoid organization, Mitochondrial nucleoid |
| الوصف: | Mitochondria, the only semiautonomous organelles in mammalian cells, possess a circular, double-stranded genome termed mitochondrial DNA (mtDNA). While nuclear genomic DNA compaction, chromatin compartmentalization and transcription are known to be regulated by phase separation, how the mitochondrial nucleoid, a highly compacted spherical suborganelle, is assembled and functions is unknown. Here we assembled mitochondrial nucleoids in vitro and show that mitochondrial transcription factor A (TFAM) undergoes phase separation with mtDNA to drive nucleoid self-assembly. Moreover, nucleoid droplet formation promotes recruitment of the transcription machinery via a special, co-phase separation that concentrates transcription initiation, elongation and termination factors, and retains substrates to facilitate mtDNA transcription. We propose a model of mitochondrial nucleoid self-assembly driven by phase separation, and a pattern of co-phase separation involved in mitochondrial transcriptional regulation, which orchestrates the roles of TFAM in both mitochondrial nucleoid organization and transcription. |
| تدمد: | 1545-9985 1545-9993 |
| DOI: | 10.1038/s41594-021-00671-w |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::08806843311e6614a36093bd53e151a2 https://doi.org/10.1038/s41594-021-00671-w |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........08806843311e6614a36093bd53e151a2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15459985 15459993 |
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| DOI: | 10.1038/s41594-021-00671-w |