Human hepatocellular carcinoma cell line Hep-G2 was cultivated in a tissue like structure on macroporous carriers. Here Sponceram®, a doped ceramic material, developed by Zellwerk GmbH, Germany was used either as spherical particle (size approx. 3 × 3 mm) in a fixed bed reactor (100 mL fixed bed, medorex e.K., Germany) or as thin porous discs in a rotating bed bioreactor (500mL Red Point ® Bioreactor, ZellWerk GmbH, Germany). In both cases long-term, protein-free cultivation of Hep G2 cells up to several months could be performed. For the rotating bed bioreactor tissue like structures could be detected. Carrier discs were completely covered with a more then 1 mm thick tissue layer.