Isolation of Egg and Sperm Cells from Bauhinia blakeana
| العنوان: | Isolation of Egg and Sperm Cells from Bauhinia blakeana |
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| المؤلفون: | Lu Ming Fang, Shun De Su, Hui Qiao Tian, Ren Hua Zheng, Hui Xiao |
| المصدر: | Tropical Plant Biology. 13:353-358 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Egg cell, In vitro fertilisation, Somatic cell, medicine.medical_treatment, Bauhinia × blakeana, Plant Science, Biology, medicine.disease_cause, biology.organism_classification, 01 natural sciences, Sperm, Cell biology, 03 medical and health sciences, 030104 developmental biology, Human fertilization, medicine.anatomical_structure, Pollen, Genetics, medicine, Ovule, 010606 plant biology & botany |
| الوصف: | Isolated sperm cells and egg cells provide useful materials for the study of fertilization in higher plants, under controlled conditions, in the absence of somatic tissue. Viable egg cells from Bauhinia blakeana were isolated using enzymatic digestion and mechanical dissection. Ovules were digested with enzymes for 40–50 min and then transferred to a solution without enzymes for dissection. The three cells of the egg apparatus (i.e., the egg cell and two synergids) were released from a cut ovule by pushing the micropylar region of the ovule with a dissecting needle. Typically, 7–10 egg cells were isolated within 1 h. B. blakeana pollen is bicellular and contains only a vegetative and generative cell at anthesis. Sperm cells were isolated using an in vivo–in vitro method. The isolation of B. blakeana egg and sperm cells potentially provides a basis for in vitro fertilization of this woody dicotyledonous plant and materials for molecular biological investigations of fertilization mechanisms. |
| تدمد: | 1935-9764 1935-9756 |
| DOI: | 10.1007/s12042-020-09266-z |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::03f0d8992fd96849191884bcf7465ca0 https://doi.org/10.1007/s12042-020-09266-z |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........03f0d8992fd96849191884bcf7465ca0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19359764 19359756 |
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| DOI: | 10.1007/s12042-020-09266-z |