التفاصيل البيبلوغرافية
| العنوان: |
The isolation of tetrangomycin from terrestrial Streptomyces sp. CAH29: evaluation of antioxidant, anticancer, and anti-MRSA activity |
| المؤلفون: |
Ozakin, Suleyman, Davis, Robert W., Umile, Thomas P., Pirinccioglu, Necmettin, Kizil, Murat, Celik, Gurbet, Sen, Alaattin, Minbiole, Kevin P. C., Ince, Ebru |
| بيانات النشر: |
Birkhauser Boston, 2016. |
| سنة النشر: |
2016 |
| مصطلحات موضوعية: |
prostate adenocarcinoma, antioxidant, interleukin 1beta, protein p53, cyclin D2, cyclin D1, antioxidant activity, arginine, IC50, Antimicrobial activity, stress activated protein kinase, synthetase, caspase 8, antibacterial activity, Tetrangomycin, valine, A549 cell line, epithelium basal cell, Candida albicans, fermentation medium, rhizosphere bacterium, prostate cancer cell line, antineoplastic agent, messenger RNA, mitogen activated protein kinase, methicillin resistant Staphylococcus aureus, lung alveolus epithelium, Streptomyces, unclassified drug, antiinfective agent, glutamine, cytotoxicity, enzyme binding, leucine, Raf protein, Immunosuppressive activity, protein Bax, Staphylococcus aureus, protein bcl 2, drug isolation, RNA 16S, Streptococcus pyogenes, tumor necrosis factor, Anti-MRSA, interleukin 6, biological activity, RNA sequence, antineoplastic activity, interleukin 2, Article, unindexed drug, controlled study, human, protein expression, terrestrial species, nonhuman, Cytotoxic activity, bacterial enzyme, human cell, antifungal activity, Angucylines, phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase, molecular docking, sequence homology, lung adenocarcinoma, LNCaP cell line, drug structure, nuclear magnetic resonance, concentration response, bacterial extract, Streptomyces sp cah29, interleukin 10, tyrosine |
| الوصف: |
A rhizosphere isolate Streptomyces sp. CAH29 was found to possess potent antibacterial and antifungal activity against a variety of test organisms. Based on 16S ribosomal ribonucleic acid sequence homology studies, this strain was found to be similar to Streptomyces stramineus (gene sequence similarity 99 %). The major bioactive metabolite produced by Streptomyces sp. CAH29 isolate was extracted, purified andidentified by nuclear magnetic resonance as tetrangomycin. This known anthraquinone-exhibited antimicrobial activity against Staphylococcus aureus, Streptococcus pyogenes, methicillin resistant Staphylococcus aureus and Candida albicans with inhibition zones of 14, 10, 12 and 8 mm, respectively. Docking results demonstrate that tetrangomycin has a similar mode of action and a comparable docking score to bind to the dehydrosqualene synthase (CrtM) enzyme of methicillin resistant Staphylococcus aureus compared to the current inhibitor. Hence, this suggests that tetrangomycin has a potential to be used as an anti-methicillin resistant Staphylococcus aureus agent. Tetrangomycin also showed moderate free radical scavenging activity with 1,1-diphenyl-2-picryl-hydrazil. Tetrangomycin apparently decreased all of the studied cytokine (pro-inflammatory: interleukin 1B, interleukin 2, tumor necrosis factor and interleukin L6 and anti-inflammatory: interleukin 10) expression levels at IC50 concentrations in A459 (adenocarcinomic human alveolar basal epithelial) and LNCAP (human prostate adenocarcinoma) cell lines. In addition, it reduced Caspase 8 and 3 mRNA levels in LNCAP and A549 cells. This study describes for the first time novel in vitro immunosuppressive function of tetrangomycin by reducing the transcription of cytokine genes. © 2016, Springer Science+Business Media New York. |
| اللغة: |
English |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::5185af3618c8656981e26a1923b212b3
http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/9350 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.dedup.wf.001..5185af3618c8656981e26a1923b212b3 |
| قاعدة البيانات: |
OpenAIRE |