Study on the activation mechanism of BCL-2 related ovarian killer (BOK)
| العنوان: | Study on the activation mechanism of BCL-2 related ovarian killer (BOK) |
|---|---|
| المؤلفون: | Goya Grocin, Andrea |
| المساهمون: | Terrones Urio, Oihana, Landeta Díaz, Olatz, CSIC, UPV/EHU, F. CIENCIA Y TECNOLOGIA, ZIENTZIA ETA TEKNOLOGIA F., Biokimikako eta Biologia Molekularreko Gradua, Biokimika eta Biologia Molekularreko Saila |
| المصدر: | Addi. Archivo Digital para la Docencia y la Investigación instname |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | BOK, BCL-2 family, mitochondrial apoptosis, oligomerization |
| الوصف: | BCL-2 family proteins are key regulators of the mitochondrial apoptotic machinery, controlling the mitochondrial outer membrane (MOM) permeabilization (MOMP). BCL-2 related Ovarian Killer (BOK) is a poorly understood pro-apoptotic member of this protein family. It has been reported that BOK localizes predominantly (although not exclusively) at membranes of the endoplasmic reticulum and of the Golgi apparatus. However, it is unclear whether BOK also operates at the MOM to promote apoptosis, as other pro-apoptotic BCL-2 family members do. Basing on the fact that the other two BAX-like pro-apoptotic members have been reported to oligomerize in order to induce MOMP, site-directed mutagenesis was used to generate two point mutations that predictably eliminated BOK’s oligomerization capacity. Then, the effect of such mutations on BOK’s membrane activity was examined using fluorescence spectroscopy. |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::285bd7dff917f6e68c18ba63b266bfc4 http://hdl.handle.net/10810/18102 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.dedup.wf.001..285bd7dff917f6e68c18ba63b266bfc4 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |