Periodical
The different activities of the two activation domains of the Brn-3a transcription factor are dependent on the context of the binding site.
| العنوان: | The different activities of the two activation domains of the Brn-3a transcription factor are dependent on the context of the binding site. |
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| المؤلفون: | Budhram-Mahadeo, V, Morris, P J, Lakin, N D, Dawson, S J, Latchman, D S |
| المصدر: | Journal of Biological Chemistry; April 1996, Vol. 271 Issue: 15 p9108-13, 6p |
| مستخلص: | The POU (Pit-Oct-Unc) family transcription factor Brn-3a contains two distinct activation domains, one at the N terminus of the molecule and one at the C terminus coincident with the DNA binding domain. These different activation domains have been shown previously to differ in their ability to activate an artificial test promoter containing a Brn-3a binding site and the naturally occurring alpha-internexin gene promoter. Here we identify the target site for Brn-3a in the alpha-internexin gene promoter and show that it can confer responsiveness to Brn-3a on a heterologous promoter. One of the single-stranded DNA sequences derived from either this novel Brn-3a binding site or from the previously characterized site in the test promoter are shown to bind Brn-3a preferentially compared with the complementary single strand or the corresponding double-stranded sequence. The pattern of responsiveness of these two sequences when cloned upstream of the same test promoter and co-transfected with constructs encoding various portions of Brn-3a indicates that the activity of the two Brn-3a activation domains is dependent upon differences in the context of the target sequence in each promoter rather than on differences in the target sequence itself. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00219258 1083351X |
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