Periodical
Viable Trypanosoma cruzicultured from a dead Paratriatoma lecticularia(Hemiptera: Reduviidae) encountered in a large dog kennel environment in south Texas, USA
| العنوان: | Viable Trypanosoma cruzicultured from a dead Paratriatoma lecticularia(Hemiptera: Reduviidae) encountered in a large dog kennel environment in south Texas, USA |
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| المؤلفون: | Busselman, Rachel E, Killets, Keswick C, Saunders, Ashley B, Hamer, Sarah A |
| المصدر: | Journal of Medical Entomology; January 2025, Vol. 62 Issue: 1 p225-229, 5p |
| مستخلص: | Trypanosoma cruzi(Chagas, 1909) is a protozoan parasite transmitted by triatomine (Hemiptera: Reduviidae) insects and is the causative agent of Chagas disease. Oral transmission of the parasite occurs through consumption of contaminated food or infected triatomines and may depend on the degree to which T. cruzisurvives in triatomine abdomens. Dead triatomines may be abundant in areas with insecticide use, such as dog kennels where animals may encounter them. We attempted to culture T. cruzifrom the gut material of 108 triatomines collected near dog kennels—14 found alive and 94 found dead—and also tested for T. cruziDNA and discrete typing units using PCR. In total, 30 (27.8%) tested positive for T. cruziusing PCR, 5 alive (35.7%) and 25 dead (26.6%), with no difference in infection between insects found alive versus dead (P-value = 0.53) and more PCR positives identified in dead triatomines with intact gut contents than in dead desiccated triatomines (P-value = 0.049). One Paratriatoma lecticularia(Stål, 1859) that was found dead (1.1%, n= 94) had T. cruzigrowth in culture. Given the use of bleach for external decontamination of triatomines as well as the level of bacterial and fungal contamination of cultures, both of which may have impacted the growth of T. cruzi, the apparent prevalence of viable parasites in this study should be interpreted as a conservative estimate. Vector control initiatives should consider that dead insects may still pose a risk of T. cruzitransmission to animals and humans. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00222585 19382928 |
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| DOI: | 10.1093/jme/tjae129 |