Periodical
Surface-confined persistent luminescence with amplified FRET efficiency for cell-cell communication monitoring
| العنوان: | Surface-confined persistent luminescence with amplified FRET efficiency for cell-cell communication monitoring |
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| المؤلفون: | Miao, Ziyun, Dai, Wenjing, Jin, Yubin, Qi, Bing, Chen, Jiayi, Qiu, Lingling, Wei, Yurong, Wang, Jie |
| المصدر: | SCIENCE CHINA Chemistry; 20250101, Issue: Preprints p1-11, 11p |
| مستخلص: | Microbial communication monitoring is vital for understanding the mechanisms beneath gut microbiota equilibrium, infections, and biomanufacturing. Herein, we reported monitoring bacterial communication by surface-confined persistent luminescence. Specifically, we constructed core-shell structured Zn1.4Ga1.2Ge0.4O4@Zn1.4Ga1.2Ge0.4O4:Cr (ZGGO@ZGGO:Cr) persistent luminescence nanoparticles (PLNPs) with amplified FRET efficiency for the detection of azoreductase (AzoR) that participates in bacterial communication. ZGGO@ZGGO:Cr nanoparticles were functionalized with an azo black hole quencher (ZGGO@ZGGO:Cr-BHQ-3). Due to the proximity of surface-confined persistent luminescence with the surface-bound BHQ-3, ZGGO@ZGGO:Cr-BHQ-3 exhibited a FRET efficiency up to 1.6 times that of traditional ZGGO:Cr-BHQ-3. Further, the specific catalytic reduction of azo bonds in BHQ-3 by bacterial extracellular AzoR recovers the persistent luminescence of ZGGO@ZGGO:Cr. ZGGO@ZGGO:Cr-BHQ-3 displayed superior sensitivity and speed in detecting extracellular AzoR over ZGGO:Cr-BHQ-3, attributed to its surface-confined persistent luminescence. We found that the peak secretion of AzoR occurs during the exponential growth period of bacteria including S. aureusand E. colibased on the persistent luminescence recovery rate of ZGGO@ZGGO:Cr-BHQ-3. Furthermore, ZGGO@ZGGO:Cr-BHQ-3 was used to probe the communication between different bacterial species. R. palustrisis a kind of lycopene-production bacteria. We found that the cohabitation of R. palustrisand S. putrefaciensboosted both AzoR secretion and lycopene production. Moreover, the secretion and activity of AzoR positively correlated with lycopene production, suggesting that AzoR may participate in the communication between R. palustrisand S. putrefaciensto promote lycopene production. This study offers a robust method to enhance PLNP-based biosensing sensitivity and provides a promising strategy for microbial communication monitoring. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 16747291 18691870 |
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| DOI: | 10.1007/s11426-024-2450-6 |