التفاصيل البيبلوغرافية
| العنوان: |
Protocol for functional screening of CFTR-targeted genetic therapies in patient-derived organoids using DETECTOR deep-learning-based analysis |
| المؤلفون: |
Bulcaen, Mattijs, Liu, Ronald B., Gryspeert, Kasper, Thierie, Sam, Ramalho, Anabela S., Vermeulen, François, Casadevall I Solvas, Xavier, Carlon, Marianne S. |
| المصدر: |
STAR Protocols; 20250101, Issue: Preprints |
| مستخلص: |
Here, we present a protocol for the rapid functional screening of gene editing and addition strategies in patient-derived organoids using the deep-learning-based tool DETECTOR (detection of targeted editing of cystic fibrosis transmembrane conductance regulator [CFTR] in organoids). We describe steps for wet-lab experiments, image acquisition, and CFTR function analysis by DETECTOR. We also detail procedures for applying pre-trained models and training custom models on new customized datasets. |
| قاعدة البيانات: |
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