Periodical
Investigation of OMNIgene•SPUTUM performance in delayed tuberculosis testing by smear, culture, and Xpert MTB/RIF assays in Uganda
| العنوان: | Investigation of OMNIgene•SPUTUM performance in delayed tuberculosis testing by smear, culture, and Xpert MTB/RIF assays in Uganda |
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| المؤلفون: | Kelly-Cirino, C. D., Musisi, E., Byanyima, P., Kaswabuli, S., Andama, A., Sessolo, A., Sanyu, I., Zawedde, J., Curry, P. S., Huang, L. |
| المصدر: | Journal of Epidemiology and Global Health; January 2017, Vol. 7 Issue: 2 p103-109, 7p |
| مستخلص: | OMNIgene•SPUTUM (OM-S) is a sample transport reagent designed to work with all tuberculosis diagnostics while eliminating the need for cold chain. OM-S-treated sputum samples were assayed in several tests after multiday holds. Raw sputa from 100 patients underwent direct smear microscopy, were manually split and assigned to the OM-S group [OM-S added at collection (no other processing required) and tested after 0- to 5-day holds at room temperature] or standard-of-care (SOC) group (NaOH/N-acetyl L-cysteine decontamination, all tested on day of collection). Concentrated smear microscopy, Lowenstein Jensen (LJ) culture, and mycobacteria growth indicator tube (MGIT) culture were performed. For patients with negative direct smear, a second sample was split, with SOC (raw sputum) and OM-S portions (sediment) tested in the Xpert MTB/RIF (Xpert) assay. OM-S group and SOC group results were strongly concordant on all four tests [range, 89% (MGIT)–97% (Xpert)]. OM-S MGIT, LJ, and Xpert tests were in statistical agreement with SOC MGIT as reference. OM-S specimens had lower culture contamination rates (3% vs. 10% LJ; 2% vs. 5% MGIT) but required, on average, 5.6 additional days to become MGIT-positive. The findings suggest that samples held/transported in OM-S are compatible with smear microscopy, LJ or MGIT culture, and Xpert, and perform comparably to fresh sputum samples. Larger feasibility studies are warranted. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 22106006 22106014 |
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| DOI: | 10.1016/j.jegh.2017.04.001 |