Periodical
Exploring the Immunomodulatory Properties of Red Onion (Allium cepaL.) Skin: Isolation, Structural Elucidation, and Bioactivity Study of Novel Onion Chalcones Targeting the A2AAdenosine Receptor
| العنوان: | Exploring the Immunomodulatory Properties of Red Onion (Allium cepaL.) Skin: Isolation, Structural Elucidation, and Bioactivity Study of Novel Onion Chalcones Targeting the A2AAdenosine Receptor |
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| المؤلفون: | Zhang, Rui-Jia, Rao, Qian-Ru, Jiang, Xue-Qin, Ye, Neng, Li, Na, Du, Hong-Ling, Zhang, Shun-Jie, Ye, Hao-Yu, Wu, Wen-Shuang, Zhao, Min |
| المصدر: | Journal of Agricultural and Food Chemistry; 20240101, Issue: Preprints |
| مستخلص: | Onions are versatile and nutritious food widely used in various cuisines around the world. In our ongoing pursuit of bioactive substances with health benefits from red onion (Allium cepaL.) skin, a comprehensive chemical investigation was undertaken. Consequently, a total of 44 compounds, including three previously unidentified chalcones (1–3) were extracted from red onion skin. Of these isolates, chalcones 1–4showed high affinity to A2Aadenosine receptor (A2AAR), and chalcone 2displayed the best binding affinity to A2AAR, with the IC50value of 33.5 nM, good A2AAR selectivity against A1AR, A2BAR, and A3AR, and high potency in the cAMP functional assay (IC50of 913.9 nM). Importantly, the IL-2 bioassay and the cell-mediated cytotoxicity assay demonstrated that chalcone 2could boost T-cell activation. Furthermore, the binding mechanism of chalcone 2with hA2AAR was elucidated by molecular docking. This work highlighted that the active chalcones in red onion might have the potential to be developed as A2AAR antagonists used in cancer immunotherapy. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 00218561 15205118 |
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| DOI: | 10.1021/acs.jafc.3c03851 |