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Neonatal necrotizing enterocolitis: Clostridium butyricumand Clostridium neonatalefermentation metabolism and enteropathogenicity
| العنوان: | Neonatal necrotizing enterocolitis: Clostridium butyricumand Clostridium neonatalefermentation metabolism and enteropathogenicity |
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| المؤلفون: | Ferraris, Laurent, Balvay, Aurélie, Bellet, Deborah, Delannoy, Johanne, Maudet, Claire, Larcher, Thibaut, Rozé, Jean-Christophe, Philippe, Catherine, Meylheuc, Thierry, Butel, Marie-José, Rabot, Sylvie, Aires, Julio |
| المصدر: | Gut Microbes; December 2023, Vol. 15 Issue: 1 |
| مستخلص: | ABSTRACTBacterial colonization in the gut plays a pivotal role in neonatal necrotizing enterocolitis (NEC) development, but the relationship between bacteria and NEC remains unclear. In this study, we aimed to elucidate whether bacterial butyrate end-fermentation metabolites participate in the development of NEC lesions and confirm the enteropathogenicity of Clostridium butyricumand Clostridium neonatalein NEC. First, we produced C.butyricumand C.neonatalestrains impaired in butyrate production by genetically inactivating the hbdgene encoding β-hydroxybutyryl-CoA dehydrogenase that produces end-fermentation metabolites. Second, we evaluated the enteropathogenicty of the hbd-knockout strains in a gnotobiotic quail model of NEC. The analyses showed that animals harboring these strains had significantly fewer and less intense intestinal lesions than those harboring the respective wild-type strains. In the absence of specific biological markers of NEC, the data provide original and new mechanistic insights into the disease pathophysiology, a necessary step for developing potential novel therapies. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 19490976 19490984 |
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| DOI: | 10.1080/19490976.2023.2172666 |