التفاصيل البيبلوغرافية
| العنوان: |
TERRA stability is regulated by RALY and polyadenylation in a telomere-specific manner |
| المؤلفون: |
Savoca, Valeria, Rivosecchi, Julieta, Gaiatto, Alice, Rossi, Annalisa, Mosca, Riccardo, Gialdini, Irene, Zubovic, Lorena, Tebaldi, Toma, Macchi, Paolo, Cusanelli, Emilio |
| المصدر: |
Cell Reports; April 2023, Vol. 42 Issue: 4 |
| مستخلص: |
Telomeric repeat-containing RNA (TERRA) is a long non-coding RNA transcribed from telomeres that plays key roles in telomere maintenance. A fraction of TERRA is polyadenylated, and the presence of the poly(A) tail influences TERRA localization and stability. However, the mechanisms of TERRA biogenesis remain mostly elusive. Here, we show that the stability of TERRA transcripts is regulated by the RNA-binding protein associated with lethal yellow mutation (RALY). RALY depletion results in lower TERRA levels, impaired localization of TERRA at telomeres, and ultimately telomere damage. Importantly, we show that TERRA polyadenylation is telomere specific and that RALY preferentially stabilizes non-polyadenylated TERRA transcripts. Finally, we report that TERRA interacts with the poly(A)-binding protein nuclear 1 (PABPN1). Altogether, our results indicate that TERRA stability is regulated by the interplay between RALY and PABPN1, defined by the TERRA polyadenylation state. Our findings also suggest that different telomeres may trigger distinct TERRA-mediated responses. |
| قاعدة البيانات: |
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