Periodical
Antimicrobial Resistance Profiles and Molecular Characteristics of Extended-Spectrum β-Lactamase-Producing Escherichia coliIsolated from Healthy Cattle and Pigs in Korea
| العنوان: | Antimicrobial Resistance Profiles and Molecular Characteristics of Extended-Spectrum β-Lactamase-Producing Escherichia coliIsolated from Healthy Cattle and Pigs in Korea |
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| المؤلفون: | Song, Hyun-Ju, Moon, Dong Chan, Kim, Su-Jeong, Mechesso, Abraham Fikru, Choi, Ji-Hyun, Boby, Naila, Kang, Hee Young, Na, Seok-Hyeon, Yoon, Soon-Seek, Lim, Suk-Kyung |
| المصدر: | Foodborne Pathogens & Disease; January 2023, Vol. 20 Issue: 1 p7-16, 10p |
| مستخلص: | Antimicrobial-resistant bacteria isolated from food animals pose a major health threat to the public on this planet. This study aimed to determine the susceptibility profiles of Escherichia coliisolated from cattle and pig fecal samples and investigate the molecular characteristics of extended-spectrum β-lactamase (ESBL)-producing E. coliusing gene identification, conjugation, and Southern blot approach. Overall 293 E. coliwere recovered from cattle (120 isolates) and pigs (173 isolates) in 7 provinces of Korea during 2017–2018. Ampicillin, chloramphenicol, streptomycin, and sulfisoxazole resistance rates were the highest in pigs' isolates (>60%, p≤ 0.001) compared to that in cattle (3–39%). Multidrug resistance (MDR) was higher in pig isolates (73%) than in cattle (31%), and the MDR profile usually includes streptomycin, sulfisoxazole, and tetracycline. Resistance to critically important antimicrobials such as ceftiofur, colistin, and ciprofloxacin was higher in weaners than those from finishers in pigs. The qnrSgene was detected in 13% of the pig isolates. Eight isolates from pigs and one isolate from cattle were identified as ESBL-producers and ESBL genes belonged to blaCTX-M-55(n= 4), blaCTX-M-14(n= 3), and blaCTX-M-65(n= 2). Notably, the blaCTX-M-65and qnrS1genes were found to be carried together in an identical plasmid (IncHI2) in two isolates from finisher pigs. The blaCTX-M-carrying isolates belonged to phylogenetic groups B1 (n= 4), B2 (n= 2), A (n= 2), and D (n= 1). The blaCTX-Mgenes and non-β-lactam resistance traits were transferred to the E. coliJ53 recipient from seven blaCTX-M-positive strains isolated from pigs. The blaCTX-Mgenes belonged to the IncI1α, IncFII, and IncHI2 plasmids and are also associated with the ISEcp1, IS26, IS903, and orf477elements. These findings suggested the possibility of blaCTX-M-carrying E. colitransmission to humans through direct contact with cattle and pigs or contamination of food products. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 15353141 15567125 |
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| DOI: | 10.1089/fpd.2022.0051 |