Periodical
Rescue of a paralyzed-flagella mutant of Chlamydomonas by transformation.
| العنوان: | Rescue of a paralyzed-flagella mutant of Chlamydomonas by transformation. |
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| المؤلفون: | Diener, D R, Curry, A M, Johnson, K A, Williams, B D, Lefebvre, P A, Kindle, K L, Rosenbaum, J L |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America; August 1990, Vol. 87 Issue: 15 p5739-5743, 5p |
| مستخلص: | The biflagellate alga Chlamydomonas has been used extensively in the genetic and biochemical analysis of flagellar assembly and motility. We have restored motility to a paralyzed-flagella mutant of Chlamydomonas by transforming with the corresponding wild-type gene. A nitrate reductase-deficient paralyzed-flagella strain, nit1-305 pf-14, carrying mutations in the genes for nitrate reductase and radial spoke protein 3, was transformed with wild-type copies of both genes. Two-thirds of the cells that survived nitrate selection also regained motility, indicating that they had been transformed with both the nitrate reductase and radial spoke protein 3 genes. Transformants typically contained multiple copies of both genes, genetically linked to each other, but not linked to the original mutant loci. Complementation of paralyzed-flagella mutants by transformation is a powerful tool for investigating flagellar assembly and function. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00278424 10916490 |
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| DOI: | 10.1073/pnas.87.15.5739 |