Periodical
In vivoand in silicoapproaches to assess surface water genotoxicity from Tocantins River, in the cities of Porto Nacional and Palmas, Brazil
| العنوان: | In vivoand in silicoapproaches to assess surface water genotoxicity from Tocantins River, in the cities of Porto Nacional and Palmas, Brazil |
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| المؤلفون: | Neto, José Lopes Soares, de Carli, Raíne Fogliati, Lehmann, Mauricio, de Souza, Cláudia Telles, Niekraszewicz, Liana Appel Boufleur, Dias, Johnny Ferraz, da Silva, Fernanda Rabaioli, da Silva, Juliana, Dihl, Rafael Rodrigues |
| المصدر: | Journal of Environmental Science and Health, Part C: Toxicology and Carcinogenesis; January 2022, Vol. 40 Issue: 1 p27-45, 19p |
| مستخلص: | AbstractThe main environmental problem in urban areas, especially in Brazil, is the discharge of untreated sewage. The in vivo Drosophila melanogasterSomatic Mutation and Recombination Test (SMART) was used to assess the genotoxicity of surface waters from three different sites in the Tocantins River, Brazil. The in silicoapproach was used to search for known and predicted interactions between environmental chemicals found in our samples and Drosophila and human proteins. The genotoxicity tests were performed in standard (ST) and high bioactivation (HB) crosses with samples collected at two periods, the rainy and dry seasons. Mutant spot frequencies found in treatments with unprocessed water from the test sites were compared with the frequencies observed in negative controls. The collection points were represented as sites A, B and C along Tocantins River. Sites A and B are located in Porto Nacional City, whereas site C is located in Palmas City. Considering the rainy season collection, positive responses in the ST cross were observed for sites A and C (89.47% and 85% of recombination, respectively) and in the HB cross for sites A, B and C (88.24%, 84.21% and 82.35% of recombination, respectively). The positive results in the dry season were restricted to sites A and B (88.89% and 85.71% of recombination, respectively) in the HB cross. In accordance with in vivoand in silicoresults, we hypothesize that ribosomal proteins (RPs) in fruit fly and humans are depleted in cells exposed to heavy metal causing DNA damage and chromosome instability, increasing homologous recombination. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 26896583 26896591 |
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| DOI: | 10.1080/26896583.2021.2014278 |