Periodical
Evaluation of a New Norovirus Genogroups GI and GII In VitroMolecular Diagnostic Assay Using Clinical Specimens Collected from Acute Diarrheal Outbreaks
| العنوان: | Evaluation of a New Norovirus Genogroups GI and GII In VitroMolecular Diagnostic Assay Using Clinical Specimens Collected from Acute Diarrheal Outbreaks |
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| المؤلفون: | Chiu, Shu-Chun, Hu, Szu-Chieh, Liao, Ling-Min, Chen, Yu-Hua, Liao, Hui-Wen, Cheng, Ju-Chien, Lin, Jih-Hui |
| المصدر: | Foodborne Pathogens & Disease; May 2022, Vol. 19 Issue: 5 p311-315, 5p |
| مستخلص: | Norovirus is a leading cause of acute gastroenteritis (AGE) in Taiwan. To improve diagnosis as part of laboratory surveillance, AGE surveillance was conducted using a new fluorescent probe hydrolysis-based insulated isothermal polymerase chain reaction (PCR) method, the POCKIT system, and the results were compared with those obtained from conventional methods. A total of 119 clinical stool samples from reported AGE outbreaks were collected for this study. From 83 real-time reverse transcription PCR (rRT-PCR) norovirus-positive cases, the POCKIT system identified 78 with a sensitivity of 90.3% in GI genogroup and 96.7% in GII genogroup. The specificity for both GI and GII genogroups was 100%. Overall, the POCKIT system is faster and easier to use than the conventional rRT-PCR method, and because of its high sensitivity and specificity, this system is a promising alternative for the detection of norovirus in patients with AGE, and would benefit public health laboratories for near real-time surveillance of AGE epidemic outbreaks. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 15353141 15567125 |
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| DOI: | 10.1089/fpd.2021.0097 |