Periodical
PCR probes for chromosome in situ hybridization of large-insert bacterial recombinants
| العنوان: | PCR probes for chromosome in situ hybridization of large-insert bacterial recombinants |
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| المؤلفون: | Kroisel, P.M., loannou, P.A., de Jong, P.J. |
| المصدر: | Cytogenetics and Cell Genetics; January 1994, Vol. 65 Issue: 1-2 p97-100, 4p |
| مستخلص: | We have developed a procedure for efficient in situ hybridization of bacterial recombinants created with various types of large-insert cloning vectors. Minimal quantities of crude DNA are amplified and labeled during the degenerate-oligonucleotide-primed polymerase chain reaction. The resulting probes generate high-intensity fluorescent hybridization signals on metaphase chromosomes and on interphase nuclei. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 03010171 14219816 |
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| DOI: | 10.1159/000133609 |