Periodical
Uncovering expression and functional analysis of newly discovered high-affinity K+transporter family members from sugarcane
| العنوان: | Uncovering expression and functional analysis of newly discovered high-affinity K+transporter family members from sugarcane |
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| المؤلفون: | Luo, Hai-bin, Huang, Cheng-mei, Wei, Yuan-wen, Zhu, Hui-ming, Cao, Hui-qing, Jiang, Sheng-li, Deng, Zhi-nian, Wu, Kai-chao, Xu, Lin |
| المصدر: | Journal of Plant Biochemistry and Biotechnology; December 2022, Vol. 31 Issue: 4 p826-836, 11p |
| مستخلص: | Potassium ions are widely involved in a series of physiological and biochemical processes of plants, which is of great significance to plant growth. High-affinity K+(HAK) transporter mainly absorbed and transported potassium in plants, but there are few studies on HAKgene in sugarcane. In this study, the coding region of high-affinity K+transporter genes were cloned from sugarcane and designated as ScHAK9, ScHAK10, ScHAK11(GenBank accession number: MG564720, MG564721, MG564722). Phylogenetic analysis results confirmed that ScHAK9and ScHAK11had the closest relationship with ZmHAK9and ZmHAK11(Zea mays), ScHAK10had the closest relationship with SbHAK10(Sorghum bicolor). In subcellular localization experiments, the fusion protein of ScHAK9 and ScHAK11 with green fluorescent protein was specifically localized in the cell membrane, but ScHAK10 green fluorescent protein was not detected, it was speculated to be expressed in Golgi apparatus. The gene expression level of ScHAKin different tissues of sugarcane at the growth periods was different, and the gene expression level of ScHAKgenes were up-regulated by the low-potassium and salt stress. Through the functional characterization experiments of ScHAKgenes in K+uptake-deficient yeasts, it was founded that ScHAKgenes possessed K+transporter activity. The study indicated that ScHAKgenes might mediate K+absorption through the cell membrane and might be participate in maintaining Na+/K+homeostasis in sugarcane under the adversity stress, and the development of plant organs is regulated by the potassium ions transport of ScHAKgenes. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 09717811 09741275 |
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| DOI: | 10.1007/s13562-021-00726-5 |