Transcriptome Changes in Mycoplasma hyopneumoniaeduring Infection

التفاصيل البيبلوغرافية
العنوان:	Transcriptome Changes in Mycoplasma hyopneumoniaeduring Infection
المؤلفون:	Madsen, Melissa L., Puttamreddy, Supraja, Thacker, Eileen L., Carruthers, Michael D., Minion, F. Chris
المصدر:	Infection and Immunity; February 2008, Vol. 76 Issue: 2 p658-663, 6p
مستخلص:	ABSTRACTMycoplasma hyopneumoniaecauses swine pneumonia and contributes significantly to the porcine respiratory disease complex. The mechanisms of pathogenesis are difficult to address, since there is a lack of genetic tools, but microarrays are available and can be used to study transcriptional changes that occur during disease as a way to identify important virulence-related genes. Mycoplasmas were collected from bronchial alveolar lavage samples and compared to broth-grown cells using microarrays. Bronchial alveolar lavage was performed on pigs 28 days postinfection, and mycoplasmas were isolated by differential centrifugation. Mycoplasma RNA-enriched preparations were then obtained from total RNA by subtracting eucaryotic ribosomal and messenger RNAs. Labeled cDNAs were generated with mycoplasma open reading frame-specific primers. Nine biological replicates were analyzed. During lung infection, our analysis indicated that 79 M. hyopneumoniaegenes were differentially expressed (P< 0.01), at a false-discovery rate of <2.7%. Of the down-regulated genes, 28 of 46 (61%) lacked an assigned function, in comparison to 21 of 33 (63%) of up-regulated genes. Four down-regulated genes and two up-regulated genes encoded putative lipoproteins. secA(mhp295) (P= 0.003) and two glycerol transport permease genes (potA[mhp380; P= 0.006] and ugpA[mhp381; P= 0.003]) were up-regulated in vivo. Elongation factor EF-G (fusA[mhp083]) (P= 0.002), RNA polymerase beta chain (rpoC[mhp635]) (P= 0.003), adenylate kinase (adk[mhp208]) (P= 0.001), prolyl aminoacyl tRNA synthetase (proS[mhp397]) (P= 0.009), and cysteinyl-tRNA synthetase (cysS[mhp661]) (P< 0.001) were down-regulated in vivo.
قاعدة البيانات:	Supplemental Index

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تدمد:	00199567 10985522
DOI:	10.1128/IAI.01291-07