التفاصيل البيبلوغرافية
| العنوان: |
Powdery mildew disease resistance and marker-assisted screening at the Pm60locus in wild diploid wheat Triticum urartu |
| المؤلفون: |
Zhao, Fukai, Li, Yinghui, Yang, Baoju, Yuan, Hongbo, Jin, Cong, Zhou, Lixun, Pei, Hongcui, Zhao, Lifang, Li, Yiwen, Zhou, Yilin, Xie, Jiankun, Shen, Qian-Hua |
| المصدر: |
The Crop Journal; April 2020, Vol. 8 Issue: 2 p252-259, 8p |
| مستخلص: |
Triticum urartu, a diploid wild wheat and progenitor of the A genome of bread wheat, is an important resource for resistance to powdery mildew fungus caused by Blumeria graminisf. sp. tritici(Bgt). In the present study we systematically characterized the interaction between the Bgtfungus and T. urartuat the microscopic level. We also tested 227 T. urartuaccessions for reaction to Bgtisolate E09 and discovered previously uncategorized powdery mildew resistance in this collection. Pm60is a CC-NB-LRR type powdery mildew resistance gene that has at least three functional alleles, Pm60, Pm60a, and Pm60b. A marker-assisted screen targeting the Pm60locus identified a non-functional allele of Pm60a, designated as Pm60a′. A sequence comparison of Pm60a′ and Pm60arevealed that they differed by 58 SNPs and one 3-nucleotide deletion. Based on the sequence variations two molecular markers were developed to differentiate the functional Pm60aallele from the non-functional Pm60a′. Our screen revealed the presence of a previously uncharacterized powdery mildew resistance in T. urartuand provides new insights into the Pm60locus. We believe that the two molecular markers developed here and new T. urarturesistant accessions will facilitate further identification of novel powdery mildew resistance genes and benefit breeding for powdery mildew resistance. |
| قاعدة البيانات: |
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