Periodical
Comparison of a TaqMan real-time polymerase chain reaction assay with a loop-mediated isothermal amplification assay for detection of Gallid herpesvirus 1
| العنوان: | Comparison of a TaqMan real-time polymerase chain reaction assay with a loop-mediated isothermal amplification assay for detection of Gallid herpesvirus 1 |
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| المؤلفون: | Ou, Shan-Chia, Giambrone, Joseph J., Macklin, Kenneth S. |
| المصدر: | Journal of Veterinary Diagnostic Investigation; January 2012, Vol. 24 Issue: 1 p138-141, 4p |
| مستخلص: | A TaqMan real-time polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) assay were developed to detect Gallid herpesvirus 1(GaHV-1, formerly Infectious laryngotracheitis virus). The standard curve of real-time PCR was established, and the sensitivity reached 10 copies/μl. In the current study, the conversion between viral titer and GaHV-1 genomic copy number was constructed. Six primers for LAMP assay amplified target gene at 65°C within 45 min, and the detection limit was 60 copies/μl. The 6 primers were highly specific, sensitive, and reproducible for detection of GaHV-1. Although the sensitivity of LAMP was lower than that of real-time PCR, LAMP was faster, less expensive, and did not require a thermocycler. The LAMP assay would be a viable alternative assay in diagnostic laboratories that do not employ real-time PCR technology. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 10406387 19434936 |
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| DOI: | 10.1177/1040638711427578 |