Periodical
Enhanced Interaction of Vibrio choleraeVirulence Regulators TcpP and ToxR under Oxygen-Limiting Conditions
| العنوان: | Enhanced Interaction of Vibrio choleraeVirulence Regulators TcpP and ToxR under Oxygen-Limiting Conditions |
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| المؤلفون: | Fan, Fenxia, Liu, Zhi, Jabeen, Nusrat, Birdwell, L. Dillon, Zhu, Jun, Kan, Biao |
| المصدر: | Infection and Immunity; January 2014, Vol. 82 Issue: 4 p1676-1682, 7p |
| مستخلص: | ABSTRACTVibrio choleraeis the causative agent of the diarrheal disease cholera. The ability of V. choleraeto colonize and cause disease requires the intricately regulated expression of a number of virulence factors during infection. One of the signals sensed by V. choleraeis the presence of oxygen-limiting conditions in the gut. It has been shown that the virulence activator AphB plays a key role in sensing low oxygen concentrations and inducing the transcription of another key virulence activator, TcpP. In this study, we used a bacterial two-hybrid system to further examine the effect of oxygen on different virulence regulators. We found that anoxic conditions enhanced the interaction between TcpP and ToxR, identified as the first positive regulator of V. choleraevirulence genes. We further demonstrated that the TcpP-ToxR interaction was dependent on the primary periplasmic protein disulfide formation enzyme DsbA and cysteine residues in the periplasmic domains of both ToxR and TcpP. Furthermore, we showed that in V. cholerae, an interaction between TcpP and ToxR is important for virulence gene induction. Under anaerobic growth conditions, we detected ToxR-TcpP heterodimers, which were abolished in the presence of the reducing agent dithiothreitol. Our results suggest that V. choleraemay sense intestinal anoxic signals by multiple components to activate virulence. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00199567 10985522 |
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| DOI: | 10.1128/IAI.01377-13 |