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Gene expression profiling in the fetal cardiac tissue after folate and lowdose trichloroethylene exposureThe GEO Database accession number GSE 18009, which will be released on December 01, 2009 and will provide access to all Microarray data.
| العنوان: | Gene expression profiling in the fetal cardiac tissue after folate and lowdose trichloroethylene exposureThe GEO Database accession number GSE 18009, which will be released on December 01, 2009 and will provide access to all Microarray data. |
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| المؤلفون: | Caldwell, Patricia T., Manziello, Ann, Howard, Jamie, Palbykin, Brittany, Runyan, Raymond B., Selmin, Ornella |
| المصدر: | Birth Defects Research Part A: Clinical and Molecular Teratology; February 2010, Vol. 88 Issue: 2 p111-127, 17p |
| مستخلص: | BACKGROUND: Previous studies show gene expression alterations in rat embryo hearts and cell lines that correspond to the cardioteratogenic effects of trichloroethylene TCE in animal models. One potential mechanism of TCE teratogenicity may be through altered regulation of calcium homeostatic genes with a corresponding inhibition of cardiac function. It has been suggested that TCE may interfere with the folic acidmethylation pathway in liver and kidney and alter gene regulation by epigenetic mechanisms. According to this hypothesis, folate supplementation in the maternal diet should counteract TCE effects on gene expression in the embryonic heart. APPROACH: To identify transcriptional targets altered in the embryonic heart after exposure to TCE, and possible protective effects of folate, we used DNA microarray technology to profile gene expression in embryonic mouse hearts with maternal TCE exposure and dietary changes in maternal folate. RESULTS: Exposure to low doses of TCE 10 ppb caused extensive alterations in transcripts encoding proteins involved in transport, ion channel, transcription, differentiation, cytoskeleton, cell cycle, and apoptosis. Exogenous folate did not offset the effects of TCE exposure on normal gene expression, and both high and low levels of folate produced additional significant changes in gene expression. CONCLUSIONS: A mechanism by which TCE induces a folate deficiency does not explain altered gene expression patterns in the embryonic mouse heart. The data further suggest that use of folate supplementation, in the presence of this toxin, may be detrimental and not protective of the developing embryo Birth Defects Research Part A, 2010. © 2009 WileyLiss, Inc. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 15420752 15420760 |
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| DOI: | 10.1002/bdra.20631 |