Periodical
Tumor Necrosis Factor-a Induces Vascular Endothelial Growth Factor-C Expression in Rheumatoid Synoviocytes
| العنوان: | Tumor Necrosis Factor-a Induces Vascular Endothelial Growth Factor-C Expression in Rheumatoid Synoviocytes |
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| المؤلفون: | CHA, HOON-SUK, BAE, EUN-KYUNG, KOH, JAY-HYUN, CHAI, JI-YOUNG, JEON, CHAN, AHN, KWANG-SUNG, KIM, JINSEOK, KOH, EUN-MI |
| المصدر: | Journal of Rheumatology; January 2007, Vol. 34 Issue: 1 p16-19, 4p |
| مستخلص: | OBJECTIVE: To determine the expression of vascular endothelial growth factor-C (VEGF-C) in the synovial fluid of patients with rheumatoid arthritis (RA) and to investigate the regulation of VEGF-C production by major proinflammatory cytokines in fibroblast-like synoviocytes (FLS). METHODS: The concentrations of VEGF-C, tumor necrosis factor-a (TNF-a), and interleukin 1ß (IL-1ß) were measured using an ELISA method in synovial fluids obtained from 20 patients with RA and 20 with osteoarthritis (OA). Primary cultured RA FLS were stimulated with TNF-a or IL-1ß, and the expression levels of VEGF-C mRNA and protein were assessed by quantitative real-time polymerase chain reaction and ELISA. RESULTS: Significantly higher levels of VEGF-C were found in RA synovial fluids compared to OA synovial fluids. VEGF-C levels showed a highly significant correlation with the levels of both TNF-a and IL-1ß in the synovial fluid of patients with RA. TNF-a stimulation significantly increased VEGF-C mRNA and protein expression in RA FLS in a dose-dependent manner. A tendency to increased expression of VEGF-C was also observed after IL-1ß stimulation in FLS. CONCLUSION: Overexpression of VEGF-C in FLS by stimulation with TNF-a may play an important role in the progression of synovial inflammation and hyperplasia in RA by contributing to local lymphangiogenesis and angiogenesis. |
| قاعدة البيانات: | Supplemental Index |
| تدمد: | 0315162X 14992752 |
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